In vitro transcription accurately predicts lac repressor phenotype in vivo in Escherichia coli
A multitude of studies have looked at the in vivo and in vitro behavior of the lac repressor binding to DNA and effector molecules in order to study transcriptional repression, however these studies are not always reconcilable. Here we use in vitro transcription to directly mimic the in vivo system...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2014-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/498.pdf |