Multiplexed proteomic profiling of cysteine reactivity and ligandability in human T cells

Summary: Differential amino acid reactivity with chemical probes can provide valuable information on the functionality and ligandability of proteins in native biological systems. Here, we present a quantitative, multiplexed chemical proteomic protocol for in-depth reactivity and ligandability profil...

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Bibliographic Details
Main Authors: Ekaterina V. Vinogradova, Benjamin F. Cravatt
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001659