Construction of an Unmarked Zymomonas mobilis Mutant Using a Site-Specific FLP Recombinase

Construction of an Unmarked Zymomonas mobilis Mutant Using a Site-Specific FLP Recombinase

Flippase expression was carried out in Zymomonas mobilis strain ZM4. The FRT-flanked selection marker gene was first integrated into the ZM4 chromosome by homologous recombination. The Saccharomyces cerevisiae flp gene was then introduced under the control of the ZM4 gap gene promoter (Pgap, encodin...

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Bibliographic Details
Main Authors: Shao-Lan Zou, Jie-Fang Hong, Cui Wang, Xin Jing, Min-Hua Zhang
Format: Article
Language:English
Published: University of Zagreb 2012-01-01
Series:Food Technology and Biotechnology
Subjects:
Zymomonas mobilis
homologous recombination
flippase expression
transformation
unmarked mutants
Online Access:http://hrcak.srce.hr/file/139047

Internet

http://hrcak.srce.hr/file/139047

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