Direct cell lysis for single-cell gene expression profiling

Direct cell lysis for single-cell gene expression profiling

The interest to analyze single and few cell samples is rapidly increasing. Numerous extraction protocols to purify nucleic acids are available, but most of them compromise severely on yield to remove contaminants and are therefore not suitable for the analysis of samples containing small numbers of...

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Bibliographic Details
Main Authors: David eSvec, Daniel eAndersson, Milos ePekny, Robert eSjöback, Mikael eKubista, Anders eStåhlberg
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-11-01
Series:Frontiers in Oncology
Subjects:
cell lysis
Real-Time PCR
single-cell biology
single-cell gene expression
RNA spike
DNA spike
Online Access:http://journal.frontiersin.org/Journal/10.3389/fonc.2013.00274/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fonc.2013.00274/full

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