Genome-wide activation screens to increase adeno-associated virus production

Genome-wide activation screens to increase adeno-associated virus production

We describe a genome-wide screening strategy to identify target genes whose modulation increases the capacity of a cell to produce recombinant adeno-associated viral (AAV) vector. Specifically, a single-guide RNA (sgRNA) library for a CRISPR-based genome-wide transcriptional activation screen was in...

Full description

Bibliographic Details
Main Authors: Christopher R. Barnes, Hyuncheol Lee, David S. Ojala, Kazuomori K. Lewis, Prajit Limsirichai, David V. Schaffer
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
adeno-associated virus
vector manufacturing
gene therapy
cell engineering
high-throughput screening
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253121001700

Internet

http://www.sciencedirect.com/science/article/pii/S2162253121001700

Similar Items