The efficacy and further functional advantages of random-base molecular barcodes for absolute and digital quantification of nucleic acid molecules

Abstract Accurate quantification of biomolecules in system-wide measurements is in high demand, especially for systems with limited sample amounts such as single cells. Because of this, digital quantification of nucleic acid molecules using molecular barcodes has been developed, making, e.g., transc...

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Bibliographic Details
Main Authors: Taisaku Ogawa, Kirill Kryukov, Tadashi Imanishi, Katsuyuki Shiroguchi
Format: Article
Language:English
Published: Nature Publishing Group 2017-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-13529-3