Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization

This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two t...

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Bibliographic Details
Main Authors: Giovana da Silva Padilha, José Carlos Curvelo Santana, Ranulfo Monte Alegre, Elias Basile Tambourgi
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2012-02-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002