Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli
Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria. Two systems widely used in Escherichia coli are the Red system from phage λ and RecET from the defective Rac prophage. We investigate...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2016-09-01
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| Series: | mBio |
| Online Access: | http://mbio.asm.org/cgi/content/full/7/5/e01443-16 |