Rapid sample preparation with Lyse-It® for Listeria monocytogenes and Vibrio cholerae.

Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio cholerae. The concept of Lyse-It® employs a conventional...

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Bibliographic Details
Main Authors: Tonya M Santaus, Shan Li, Paula Ladd, Amanda Harvey, Shannon Cole, O Colin Stine, Chris D Geddes
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6059484?pdf=render