COBRA-Seq: Sensitive and Quantitative Methylome Profiling

COBRA-Seq: Sensitive and Quantitative Methylome Profiling

Combined Bisulfite Restriction Analysis (COBRA) quantifies DNA methylation at a specific locus. It does so via digestion of PCR amplicons produced from bisulfite-treated DNA, using a restriction enzyme that contains a cytosine within its recognition sequence, such as TaqI. Here, we introduce COBRA-s...

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Bibliographic Details
Main Authors: Hilal Varinli, Aaron L. Statham, Susan J. Clark, Peter L. Molloy, Jason P. Ross
Format: Article
Language:English
Published: MDPI AG 2015-10-01
Series:Genes
Subjects:
COBRA
DNA methylation
reduced representation
non CpG
non-model organism
restriction enzymes
next generation sequencing
enhancer
CHH
Online Access:http://www.mdpi.com/2073-4425/6/4/1140

Internet

http://www.mdpi.com/2073-4425/6/4/1140

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