Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

<p>Abstract</p> <p>Background</p> <p>The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections...

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Bibliographic Details
Main Authors: Hall Leo T, Ayoubi Patricia, Macwana Sunita, Ray Anamika, Prade Rolf, Mort Andrew J
Format: Article
Language:English
Published: BMC 2004-03-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/5/22