Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells

Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells

Recombinant human pancreatic lipase-related protein 2 (rHPLRP2) was produced in the protease A-deficient yeast Pichia pastoris. A major protein with a molecular mass of 50 kDa was purified from the culture medium using SP-Sepharose and Mono Q chromatography. The protein was found to be highly sensit...

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Bibliographic Details
Main Authors: Cécilia Eydoux, Josiane De Caro, Francine Ferrato, Paul Boullanger, Dominique Lafont, René Laugier, Frédéric Carrière, Alain De Caro
Format: Article
Language:English
Published: Elsevier 2007-07-01
Series:Journal of Lipid Research
Subjects:
constitutive expression
Pichia pastoris
inhibition
galactolipid
lid domain
galactolipase
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520425386

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http://www.sciencedirect.com/science/article/pii/S0022227520425386

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