Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

Summary: In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component proteins and assembly of partially melted and fully melte...

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Bibliographic Details
Main Authors: Sergio E. Martinez, Anupam Singh, Brent De Wijngaert, Shemaila Sultana, Chhaya Dharia, Hans Vanbuel, Jiayu Shen, Daniel Vasilchuk, Smita S. Patel, Kalyan Das
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Protein expression and purification
Structural Biology
Cryo-EM
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001386

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http://www.sciencedirect.com/science/article/pii/S2666166721001386

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