Tunable translational control using site-specific unnatural amino acid incorporation in Escherichia coli
Translation of target gene transcripts in Escherichia coli harboring UAG amber stop codons can be switched on by the amber-codon-specific incorporation of an exogenously supplied unnatural amino acid, 3-iodo-L-tyrosine. Here, we report that this translational switch can control the translational eff...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2015-04-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/904.pdf |