Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmenta...

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Main Authors:	Ramunas Stepanauskas, Elizabeth A. Fergusson, Joseph Brown, Nicole J. Poulton, Ben Tupper, Jessica M. Labonté, Eric D. Becraft, Julia M. Brown, Maria G. Pachiadaki, Tadas Povilaitis, Brian P. Thompson, Corianna J. Mascena, Wendy K. Bellows, Arvydas Lubys
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-017-00128-z

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spelling	doaj-1103091ab4b04fd8bf75b654668bcb212021-01-31T12:40:20ZengNature Publishing GroupNature Communications2041-17232017-07-018111010.1038/s41467-017-00128-zImproved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particlesRamunas Stepanauskas0Elizabeth A. Fergusson1Joseph Brown2Nicole J. Poulton3Ben Tupper4Jessica M. Labonté5Eric D. Becraft6Julia M. Brown7Maria G. Pachiadaki8Tadas Povilaitis9Brian P. Thompson10Corianna J. Mascena11Wendy K. Bellows12Arvydas Lubys13Bigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesThermo Fisher Scientific BalticsBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesBigelow Laboratory for Ocean SciencesThermo Fisher Scientific BalticsSingle-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.https://doi.org/10.1038/s41467-017-00128-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys
spellingShingle	Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles Nature Communications
author_facet	Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys
author_sort	Ramunas Stepanauskas
title	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_short	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_full	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_fullStr	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_full_unstemmed	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_sort	improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-07-01
description	Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.
url	https://doi.org/10.1038/s41467-017-00128-z
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Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

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