Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmenta...

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Bibliographic Details
Main Authors: Ramunas Stepanauskas, Elizabeth A. Fergusson, Joseph Brown, Nicole J. Poulton, Ben Tupper, Jessica M. Labonté, Eric D. Becraft, Julia M. Brown, Maria G. Pachiadaki, Tadas Povilaitis, Brian P. Thompson, Corianna J. Mascena, Wendy K. Bellows, Arvydas Lubys
Format: Article
Language:English
Published: Nature Publishing Group 2017-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00128-z