Structure-based engineering of heparinase I with improved specific activity for degrading heparin

Structure-based engineering of heparinase I with improved specific activity for degrading heparin

Abstract Background Heparinase I from Pedobacter heparinus (Ph-HepI), which specifically cleaves heparin and heparan sulfate, is one of the most extensively studied glycosaminoglycan lyases. Enzymatic degradation of heparin by heparin lyases not only largely facilitates heparin structural analysis b...

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Bibliographic Details
Main Authors: Chuan Zhang, Bao-Cheng Yang, Wen-Ting Liu, Zhong-Yuan Li, Ya-Jian Song, Tong-Cun Zhang, Xue-Gang Luo
Format: Article
Language:English
Published: BMC 2019-08-01
Series:BMC Biotechnology
Subjects:
Heparinase I
Specific activity
Site-directed mutagenesis
Homology modeling
Molecular docking
Online Access:http://link.springer.com/article/10.1186/s12896-019-0553-3

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http://link.springer.com/article/10.1186/s12896-019-0553-3

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