An Optimized Protocol for ChIP-Seq from Human Embryonic Stem Cell Cultures

Summary: Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-spe...

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Bibliographic Details
Main Authors: Adrienne E. Sullivan, Silvia D.M. Santos
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300496