Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy
Analysis of side-chain motions by NMR has so far been restricted to small proteins and methyl-bearing side chains. Here, the authors present NMR methods based on 13C direct detection of highly deuterated protein samples that yield sharp and well-resolved signals and allow the characterisation of sid...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09743-4 |