Using Combined Methods of Genetic Mapping and Nanopore-Based Sequencing Technology to Analyze the Insertion Positions of G10evo-EPSPS and Cry1Ab/Cry2Aj Transgenes in Maize

Using Combined Methods of Genetic Mapping and Nanopore-Based Sequencing Technology to Analyze the Insertion Positions of G10evo-EPSPS and Cry1Ab/Cry2Aj Transgenes in Maize

The insertion position of the exogenous fragment sequence in a genetically modified organism (GMO) is important for the safety assessment and labeling of GMOs. SK12-5 is a newly developed transgenic maize line transformed with two trait genes [i.e., G10evo-5-enolpyrul-shikimate-3-phosphate synthase...

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Bibliographic Details
Main Authors: Cheng Peng, Yingting Mei, Lin Ding, Xiaofu Wang, Xiaoyun Chen, Junmin Wang, Junfeng Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-07-01
Series:Frontiers in Plant Science
Subjects:
nanopore-sequencing
next-generation sequencing
genetically modified organism
insertion position
genetic mapping
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.690951/full

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https://www.frontiersin.org/articles/10.3389/fpls.2021.690951/full

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