Comparative Analysis of Click Chemistry Mediated Activity-Based Protein Profiling in Cell Lysates

Comparative Analysis of Click Chemistry Mediated Activity-Based Protein Profiling in Cell Lysates

Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used...

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Bibliographic Details
Main Authors: Yinliang Yang, Xiaomeng Yang, Steven H. L. Verhelst
Format: Article
Language:English
Published: MDPI AG 2013-10-01
Series:Molecules
Subjects:
activity-based probes
cathepsins
click chemistry
proteases
protein modification
Online Access:http://www.mdpi.com/1420-3049/18/10/12599

Internet

http://www.mdpi.com/1420-3049/18/10/12599

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