Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells

Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cells

Previous gene editing in haematopoietic stem cells (HSCs) has focussed on a heterogeneous CD34+ population. Here, the authors demonstrate high efficiency CRISPR/Cas9-based editing of purified long-term HSCs using non-homologous end joining and homology-directed repair, by directing isoform-specific...

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Bibliographic Details
Main Authors: Elvin Wagenblast, Maria Azkanaz, Sabrina A. Smith, Lorien Shakib, Jessica L. McLeod, Gabriela Krivdova, Joana Araújo, Leonard D. Shultz, Olga I. Gan, John E. Dick, Eric R. Lechman
Format: Article
Language:English
Published: Nature Publishing Group 2019-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12726-0

Internet

https://doi.org/10.1038/s41467-019-12726-0

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