Detection of E. coli isolated from cheese by using virulence factors by PCR technique in AL-Diwaniyah city
The study was conducted to detection of Enterohemorrhagic Escherichia coli (EHEC) by important virulence factors genes such as hemolysin toxin genes (hlyA and hlyB) by using PCR technique from cattle milk product (cheese). Escherichia coli is an important bacterium that can cause foodborne disease,...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine
2016-06-01
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| Series: | Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| Online Access: | http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/380 |
| Summary: | The study was conducted to detection of Enterohemorrhagic Escherichia coli (EHEC) by important virulence factors genes such as hemolysin toxin genes (hlyA and hlyB) by using PCR technique from cattle milk product (cheese). Escherichia coli is an important bacterium that can cause foodborne disease, the raw milk, milk products, and undercooked ground meat products are the primary sources of outbreaks. A total 50 cheese sample was collected from the local market in Al-Diwaniyah city the study was done in a period between August and October 2014. The PCR primers for hlyA and hlyB gene were designed by this study from NCBI-Genbank published sequence. The PCR results were show (38) positive isolates out of 50 isolates at (76%), (23) isolates producing hlyA gene and (15) isolates producing hlyB gene whereas (9) of positive isolates were producing both hemolysin toxin genes. In conclusion, the PCR was appeared very sensitive and highly specific assay serve as a suitable molecular diagnostic tool for detection Enterohemorrhagic Escherichia coli producing hemolysin toxins. |
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| ISSN: | 1818-5746 2313-4429 |