Expression and purification of the Sgm protein from E. coli

The Sgm gene from Micromonospora zionensis, the producer of the aminoglycoside antibiotic G-52, encodes for Sgmmethylasewhich modifies the target site on 16S rRNAand thus protects the producer against its own toxic product. The sgm gene wasmodified by polymerase chain reaction (PCR) and cloned in th...

Full description

Bibliographic Details
Main Authors: Ilić-Tomić Tatjana, Marković Sandra, Vasiljević Branka Z.
Format: Article
Language:English
Published: Serbian Chemical Society 2005-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390506817I.pdf