Expression and purification of the Sgm protein from E. coli
The Sgm gene from Micromonospora zionensis, the producer of the aminoglycoside antibiotic G-52, encodes for Sgmmethylasewhich modifies the target site on 16S rRNAand thus protects the producer against its own toxic product. The sgm gene wasmodified by polymerase chain reaction (PCR) and cloned in th...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Serbian Chemical Society
2005-01-01
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Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390506817I.pdf |