A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine

A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine

There is an increased demand for comprehensive analysis of vitamin D metabolites. This is a major challenge, especially for 1α,25-dihydroxyvitamin D [1α,25(OH)2VitD], because it is biologically active at picomolar concentrations. 4-Phenyl-1,2,4-triazoline-3,5-dione (PTAD) was a revolutionary reagent...

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Bibliographic Details
Main Authors: Debin Wan, Jun Yang, Bogdan Barnych, Sung Hee Hwang, Kin Sing Stephen Lee, Yongliang Cui, Jun Niu, Mitchell A. Watsky, Bruce D. Hammock
Format: Article
Language:English
Published: Elsevier 2017-04-01
Series:Journal of Lipid Research
Subjects:
1,25-dihydroxyvitamin D
major vitamin D metabolites
matrix effect
smaller sample volume
quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520338566

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http://www.sciencedirect.com/science/article/pii/S0022227520338566

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