Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia

Summary: This protocol establishes a tri-culture of hiPSC-derived neurons, astrocytes, and microglia for the study of cellular interactions during homeostasis, injury, and disease. This system allows for mechanistic studies that can identify the roles of individual cell types in disease and injury r...

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Bibliographic Details
Main Authors: Sean K. Ryan, Kelly L. Jordan-Sciutto, Stewart A. Anderson
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301775