Reversed phase parallel artificial membrane permeation assay for log P measurement
A reversed phase parallel artificial membrane permeation assay (RP-PAMPA) was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Association of Physical Chemists (IAPC)
2016-03-01
|
| Series: | ADMET and DMPK |
| Online Access: | http://pub.iapchem.org/ojs/index.php/admet/article/view/277 |
| Summary: | A reversed phase parallel artificial membrane permeation assay (RP-PAMPA) was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between log P and the apparent permeability was observed in the -0.24 < log P < 2.85 region (R2 = 0.98). RP-PAMPA was also applied to pKa measurement. |
|---|---|
| ISSN: | 1848-7718 |