Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells [version 2; referees: 2 approved]
Background: We wanted to investigate the physical state of biological membranes in live cells under the most physiological conditions possible. Methods: For this we have been using laurdan, C-laurdan or M-laurdan to label a variety of cells, and a biphoton microscope equipped with both a thermostati...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
F1000 Research Ltd
2017-08-01
|
Series: | F1000Research |
Subjects: | |
Online Access: | https://f1000research.com/articles/6-763/v2 |