Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells [version 2; referees: 2 approved]

Background: We wanted to investigate the physical state of biological membranes in live cells under the most physiological conditions possible. Methods: For this we have been using laurdan, C-laurdan or M-laurdan to label a variety of cells, and a biphoton microscope equipped with both a thermostati...

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Bibliographic Details
Main Authors: Serge Mazeres, Farzad Fereidouni, Etienne Joly
Format: Article
Language:English
Published: F1000 Research Ltd 2017-08-01
Series:F1000Research
Subjects:
Online Access:https://f1000research.com/articles/6-763/v2