Soluble variants of human recombinant glutaminyl cyclase.

Soluble variants of human recombinant glutaminyl cyclase.

Recombinant human Glutaminyl Cyclase expressed in E. coli is produced as inclusion bodies. Lack of glycosylation is the main origin of its accumulation in insoluble aggregates. Mutation of single isolated hydrophobic amino acids into negative amino acids was not able to circumvent inclusion bodies f...

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Bibliographic Details
Main Authors: Cristiana Castaldo, Silvia Ciambellotti, Raquel de Pablo-Latorre, Daniela Lalli, Valentina Porcari, Paola Turano
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977104/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977104/?tool=EBI

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