Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system.

Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system.

Protein-protein interactions can be studied in vitro, e.g. with bacterial or yeast two-hybrid systems or surface plasmon resonance. In contrast to in vitro techniques, in vivo studies of protein-protein interactions allow examination of spatial and temporal behavior of such interactions in their nat...

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Bibliographic Details
Main Authors: Ruud G J Detert Oude Weme, Ákos T Kovács, Sander J G de Jong, Jan-Willem Veening, Jeroen Siebring, Oscar P Kuipers
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4401445?pdf=render

Internet

http://europepmc.org/articles/PMC4401445?pdf=render

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