Phage-assisted continuous evolution of proteases with altered substrate specificity
Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence specificities.
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017-10-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-01055-9 |