Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader

Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader

Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-s...

Full description

Bibliographic Details
Main Authors:	Manisha Ray, Kazufumi Nagai, Yasuyuki Kihara, Amanda Kussrow, Michael N. Kammer, Aaron Frantz, Darryl J. Bornhop, Jerold Chun
Format:	Article
Language:	English
Published:	Elsevier 2020-08-01
Series:	Journal of Lipid Research
Subjects:	receptor binding assay G protein-coupled receptor lysophospholipids molecular interaction interferometry free-solution assay-compensated interferometric reader
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520434923

Similar Items

Lysophosphatidic acid (LPA)-antibody (504B3) engagement detected by interferometry identifies off-target binding
by: Manisha Ray, et al.
Published: (2021-04-01)

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity
by: Jasmeet Singh, et al.
Published: (2012-09-01)

Lysophospholipid G protein-coupled receptor binding parameters as determined by backscattering interferometry[S]
by: Hirotaka Mizuno, et al.
Published: (2019-01-01)

Three Dimensional Interferometric Imaging at Terahertz Frequency for Concealed Object Detection
by: Goltsman, Alexander Mark
Published: (2014)

Challenges and Inconsistencies in Using Lysophosphatidic Acid as a Biomarker for Ovarian Cancer
by: Tsukasa Yagi, et al.
Published: (2019-04-01)

A characterization of the human G protein-coupled receptor, lysophosphatidic acid1 : its intracellular trafficking and signaling consequences on the tumor suppressor, P53
by: Murph, Mandi Michelle
Published: (2005)

Interferometric Synthetic Aperture Sonar Signal Processing for Autonomous Underwater Vehicles Operating Shallow Water
by: Giardina, Patricia E
Published: (2012)

Characterisation of the vascular angiotensin receptor
by: McQueen, J.
Published: (1986)

Development of single-particle counting assays with interferometric reflectance imaging
by: Ekiz Kanik, Fulya
Published: (2020)

Lysophosphatidic Acid Level and the Incidence of Silent Brain Infarction in Patients with Nonvalvular Atrial Fibrillation
by: Li Zhou, et al.
Published: (2010-10-01)

An Optimized Colorimetric Readout Method for Lateral Flow Immunoassays
by: Jongwon Park
Published: (2018-11-01)

AIRBORNE INTERFEROMETRIC SYNTHETIC APERTURE RADAR
by: E. E. Nechaev, et al.
Published: (2016-11-01)

Critical Roles of Lysophospholipid Receptors in Activation of Neuroglia and Their Neuroinflammatory Responses
by: Bhakta Prasad Gaire, et al.
Published: (2021-07-01)

The Expression Profiles of Lysophospholipid Receptors (LPLRs) in Different Endothelial Cells
by: Yu-Wei Lee, et al.
Published: (2006-03-01)

Altered cleavage plane orientation with increased genomic aneuploidy produced by receptor-mediated lysophosphatidic acid (LPA) signaling in mouse cerebral cortical neural progenitor cells
by: Whitney S. McDonald, et al.
Published: (2020-12-01)

Fibroblastic reticular cell-derived lysophosphatidic acid regulates confined intranodal T-cell motility
by: Akira Takeda, et al.
Published: (2016-02-01)

Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays
by: Popović Nataša, et al.
Published: (2009-01-01)

Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays
by: CONSTANTINOS DEMONACOS, et al.
Published: (2009-03-01)

Cell type‐selective pathways and clinical associations of lysophosphatidic acid biosynthesis and signaling in the ovarian cancer microenvironment
by: Silke Reinartz, et al.
Published: (2019-02-01)

ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions
by: Petra Pazderova, et al.
Published: (2020-06-01)

Experimental study on the role of lysophosphatidic acid in mediating cytokine/chemokine production and on the signaling pathways involved in inflammatory arthritis
by: Hui, Weili
Published: (2017)

Studies on the identification and function of metabolites involved in peroxisome proliferator-activated receptor (PPAR) α activation
by: Takahashi, Haruya
Published: (2014)

Human Astrovirus 1–8 Seroprevalence Evaluation in a United States Adult Population
by: Lena Meyer, et al.
Published: (2021-05-01)

Inhibition of Estrogenic Response of Yeast Screen Assay by Exposure to Non-Lethal Levels of Metallic Nanoparticles
by: Byoung-Cheun Lee, et al.
Published: (2020-05-01)

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays
by: Laia Reverté, et al.
Published: (2014-11-01)

Design of Chirped Gratings Using Interferometric Lithography
by: Steve Benoit, et al.
Published: (2018-01-01)

A little on EDG : the role of the cytoplasmic tail in the function of the LPA₁/Edg2 G protein coupled receptor
by: Severin, Jamie Elizabeth
Published: (2008)

Using apriori information in interferometric processing of high resolution sar data
by: V. A. Ushenkin, et al.
Published: (2016-07-01)

Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters
by: Anna Michalczyk, et al.
Published: (2017-07-01)

Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System
by: Sarah E. Mann, et al.
Published: (2020-04-01)

Presence of cerebrospinal fluid antibodies associated with autoimmune encephalitis of humans in dogs with neurologic disease
by: Emma G. Stafford, et al.
Published: (2019-09-01)

Development of a M9‐based urea medium (M9U) for sensitive and real‐time monitoring of ureolytic activity of bacteria and cell‐free urease
by: Jens Jakob Sigurdarson, et al.
Published: (2020-03-01)

SURE-Fuse WFF: A Multi-Resolution Windowed Fourier Analysis for Interferometric Phase Denoising
by: Joshin P. Krishnan, et al.
Published: (2019-01-01)

Role of phospholipase D in agonist-stimulated lysophosphatidic acid synthesis by ovarian cancer cells
by: Céline Luquain, et al.
Published: (2003-10-01)

Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis
by: Toshihiko Tsutsumi, et al.
Published: (2015-01-01)

Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids
by: Sayoko Ito-Harashima, et al.
Published: (2020-05-01)

A critical assessment of the estrogenic potency of benzyl salicylate
by: Andreas Natsch, et al.
Published: (2021-01-01)

القارئ في المناهج النقدية المعاصرة
by: حسين تروش
Published: (2016-12-01)

Regional Recognition and Classification of Active Loess Landslides Using Two-Dimensional Deformation Derived from Sentinel-1 Interferometric Radar Data
by: Qingkai Meng, et al.
Published: (2020-05-01)

Expansion Design of Interferometric Aperture Synthesis Arrays Based on Multi-Objective Optimization
by: Dong Zhu, et al.
Published: (2019-01-01)

Cannot write session to /tmp/vufind_sessions/sess_84kp0lp651lvqv4gj97ckt4po3