Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader

Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-s...

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Bibliographic Details
Main Authors: Manisha Ray, Kazufumi Nagai, Yasuyuki Kihara, Amanda Kussrow, Michael N. Kammer, Aaron Frantz, Darryl J. Bornhop, Jerold Chun
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520434923