Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts

Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts

Antibodies have been used for basic research, clinical diagnostics, and therapeutic applications. <i>Escherichia coli</i> is one of the organisms of choice for the production of recombinant antibodies. Variable antibody genes have canonical and non-canonical disulfide bonds that are form...

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Bibliographic Details
Main Authors: Bhupal Ban, Maya Sharma, Jagathpala Shetty
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Antibodies
Subjects:
recombinant antibodies
inclusion bodies
refolding
redox pair
disulfide bonds
Online Access:https://www.mdpi.com/2073-4468/9/3/39

Internet

https://www.mdpi.com/2073-4468/9/3/39

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