A modified method for genomic DNA extraction from the fish intestinal microflora

A modified method for genomic DNA extraction from the fish intestinal microflora

Abstract A modified genomic DNA extraction method named the combination of lysozyme and ultrasonic lysis (CLU) method was used to analyze the fish intestinal microflora. In this method, the physical disruption and chemical lysis steps were combined, and some parameters in the key steps were adjusted...

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Bibliographic Details
Main Authors: Zhuoran Han, Jingfeng Sun, Aijun Lv, YeongYik Sung, Xueliang Sun, Hongyue Shi, Xiucai Hu, Anli Wang, Kezhi Xing
Format: Article
Language:English
Published: SpringerOpen 2018-04-01
Series:AMB Express
Subjects:
Modified method
Genomic DNA extraction
Fish intestinal microflora
OD260/OD280 ratio
DNA concentration
Online Access:http://link.springer.com/article/10.1186/s13568-018-0578-3

Internet

http://link.springer.com/article/10.1186/s13568-018-0578-3

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