Quantitative determination of lysolecithin and sphingomyelin in phospholipid mixtures by thin-layer chromatography as applied to the thymus of the fmfm mouse
Lysolecithin and sphingomyelin may be readily separated from other phospholipids on thin-layer plates impregnated with silver nitrate using the solvent system chloroform–methanol–concentrated ammonium hydroxide 65:35:8. Quantitation is carried out by determining the phosphorus content of the develop...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1974-05-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368085 |