Quantitative determination of lysolecithin and sphingomyelin in phospholipid mixtures by thin-layer chromatography as applied to the thymus of the fmfm mouse

Lysolecithin and sphingomyelin may be readily separated from other phospholipids on thin-layer plates impregnated with silver nitrate using the solvent system chloroform–methanol–concentrated ammonium hydroxide 65:35:8. Quantitation is carried out by determining the phosphorus content of the develop...

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Bibliographic Details
Main Authors: Linda M. Hoffman, Wai Fok, Larry Schneck
Format: Article
Language:English
Published: Elsevier 1974-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520368085