K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

Abstract Background K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is presented. The protocol was accompanied by software developed to aid with pr...

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Bibliographic Details
Main Authors: Peio Ziarsolo, Tomas Hasing, Rebeca Hilario, Victor Garcia-Carpintero, Jose Blanca, Aureliano Bombarely, Joaquin Cañizares
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Plant Methods
Subjects:
Klenow polymerase
K-seq
K-mer
Low cost
SNP
Genotyping
Online Access:https://doi.org/10.1186/s13007-021-00733-6

Internet

https://doi.org/10.1186/s13007-021-00733-6

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