Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

<p>Abstract</p> <p>Background</p> <p>The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting...

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Bibliographic Details
Main Authors: Ivell Richard, Mueller Nadine, Spiess Andrej-Nikolai
Format: Article
Language:English
Published: BMC 2003-11-01
Series:BMC Genomics
Subjects:
RNA
amplification
bias
T7 Polymerase
microarray
Online Access:http://www.biomedcentral.com/1471-2164/4/44

Internet

http://www.biomedcentral.com/1471-2164/4/44

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