A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy

A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy

This study designs and accomplishes a high precision and robust laser-based autofocusing system, in which a biased image plane is applied. In accordance to the designed optics, a cluster-based circle fitting algorithm is proposed to calculate the radius of the detecting spot from the reflected laser...

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Main Authors: Chao-Chen Gu, Hao Cheng, Kai-Jie Wu, Liang-Jun Zhang, Xin-Ping Guan
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Journal of Sensors
Online Access:http://dx.doi.org/10.1155/2018/8542680
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spelling doaj-05f4ad0fff8c46f8b9e1c97c8c2b374a2020-11-25T00:00:38ZengHindawi LimitedJournal of Sensors1687-725X1687-72682018-01-01201810.1155/2018/85426808542680A High Precision Laser-Based Autofocus Method Using Biased Image Plane for MicroscopyChao-Chen Gu0Hao Cheng1Kai-Jie Wu2Liang-Jun Zhang3Xin-Ping Guan4Department of Automation, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Automation, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Automation, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Automation, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Automation, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, ChinaThis study designs and accomplishes a high precision and robust laser-based autofocusing system, in which a biased image plane is applied. In accordance to the designed optics, a cluster-based circle fitting algorithm is proposed to calculate the radius of the detecting spot from the reflected laser beam as an essential factor to obtain the defocus value. The experiment conduct on the experiment device achieved novel performance of high precision and robustness. Furthermore, the low demand of assembly accuracy makes the proposed method a low-cost and realizable solution for autofocusing technique.http://dx.doi.org/10.1155/2018/8542680
collection DOAJ
language English
format Article
sources DOAJ
author Chao-Chen Gu
Hao Cheng
Kai-Jie Wu
Liang-Jun Zhang
Xin-Ping Guan
spellingShingle Chao-Chen Gu
Hao Cheng
Kai-Jie Wu
Liang-Jun Zhang
Xin-Ping Guan
A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
Journal of Sensors
author_facet Chao-Chen Gu
Hao Cheng
Kai-Jie Wu
Liang-Jun Zhang
Xin-Ping Guan
author_sort Chao-Chen Gu
title A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
title_short A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
title_full A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
title_fullStr A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
title_full_unstemmed A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy
title_sort high precision laser-based autofocus method using biased image plane for microscopy
publisher Hindawi Limited
series Journal of Sensors
issn 1687-725X
1687-7268
publishDate 2018-01-01
description This study designs and accomplishes a high precision and robust laser-based autofocusing system, in which a biased image plane is applied. In accordance to the designed optics, a cluster-based circle fitting algorithm is proposed to calculate the radius of the detecting spot from the reflected laser beam as an essential factor to obtain the defocus value. The experiment conduct on the experiment device achieved novel performance of high precision and robustness. Furthermore, the low demand of assembly accuracy makes the proposed method a low-cost and realizable solution for autofocusing technique.
url http://dx.doi.org/10.1155/2018/8542680
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AT haocheng ahighprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT kaijiewu ahighprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT liangjunzhang ahighprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT xinpingguan ahighprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT chaochengu highprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT haocheng highprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT kaijiewu highprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT liangjunzhang highprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
AT xinpingguan highprecisionlaserbasedautofocusmethodusingbiasedimageplaneformicroscopy
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