A High Precision Laser-Based Autofocus Method Using Biased Image Plane for Microscopy

This study designs and accomplishes a high precision and robust laser-based autofocusing system, in which a biased image plane is applied. In accordance to the designed optics, a cluster-based circle fitting algorithm is proposed to calculate the radius of the detecting spot from the reflected laser...

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Bibliographic Details
Main Authors: Chao-Chen Gu, Hao Cheng, Kai-Jie Wu, Liang-Jun Zhang, Xin-Ping Guan
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Journal of Sensors
Online Access:http://dx.doi.org/10.1155/2018/8542680