Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan

• Main Authors: Nafise Sadat Majidi, Giti Emtiazi, Samaneh Shahrokh Esfahani
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2016-09-01
• Series: Journal of Medical Bacteriology
• Subjects: Chitosan; Chitin; TPP; Antibacterial activity; Flow cytometry
• Online Access: http://jmb.tums.ac.ir/index.php/jmb/article/view/188
• ISSN: 2251-8649; 2322-2581

<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong>  Chitosan is unbranched polysaccharide composed of D-glucosamine and N-acetyl-D-glucosamine. Chitosan, derived from shrimp shell, has broad antimicrobial properties against Gram-negative, Gram-positive bacteria and fungi.</p><p> </p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> Chitosan was extracted from shrimp shell and studied for cell entrapment and anti-bacterial properties. The hydrogel chitosan was used as the beads for cell entrapment and chitosan beads were designed to deliver cells and nutrients. These data confirmed with flow cytometric analyses.                </p><p> </p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> Experimental results exhibited that internal diffusion through the chitosan matrix was the main mechanism for whole gelation by TPP (Tri-polyphosphate). The minimum inhibitory concentration (MIC) for chitosan against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> was 16 and 32 μg/ml respectively.</p><p> </p></td></tr></tbody></table></div><strong><em>Conclusion</em>:<em> </em></strong> Despite the antimicrobial properties of chitosan, trapped bacteria in the gel network were alive and were chelated indicating that their access to the outside was limited.