Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting.

Western blotting has been a key technique for determining the relative expression of proteins within complex biological samples since the first publications in 1979. Recent developments in sensitive fluorescent labels, with truly quantifiable linear ranges and greater limits of detection, have allow...

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Bibliographic Details
Main Authors: Samantha L Eaton, Sarah L Roche, Maica Llavero Hurtado, Karla J Oldknow, Colin Farquharson, Thomas H Gillingwater, Thomas M Wishart
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3758299?pdf=render