A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription PCR

A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription PCR

To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of...

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Bibliographic Details
Main Authors: Tatsuhiko eHoshino, Fumio eInagaki
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-06-01
Series:Frontiers in Microbiology
Subjects:
Reverse Transcriptase Polymerase Chain Reaction
16S rRNA
Microbial Diversity
hydrothermal sediment
bias-free diversity analysis
poly(A) tailing
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00160/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00160/full

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