Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations

Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations

Summary: Here, we describe a generic protocol for monitoring protein-RNA interaction using a cleavable GFP fusion of a recombinant RNA-binding protein. We detail each expression and purification step, including high salt and heparin column for contaminant RNA removal. After the assembly of RNA into...

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Bibliographic Details
Main Authors: Yunrong Gao, Dongdong Cao, Shristi Pawnikar, Sana Akhter, Yinglong Miao, Bo Liang
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Protein biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000228

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http://www.sciencedirect.com/science/article/pii/S2666166721000228

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