The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of...

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Bibliographic Details
Main Authors: Jueeli D. Vaidya, Bartholomeus van den Bogert, Joan E. Edwards, Jos Boekhorst, Sanne van Gastelen, Edoardo Saccenti, Caroline M. Plugge, Hauke Smidt
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-01-01
Series:Frontiers in Microbiology
Subjects:
DNA extraction methods
rumen fluid
fibrous content
bacteria
archaea
fungi
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2018.00092/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2018.00092/full

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