Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves

Summary: The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method ap...

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Bibliographic Details
Main Authors: Hirosato Kanda, Sotatsu Tonomura, Yi Dai, Jianguo G. Gu
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302537