Linear Behavior of the Phase Lifetime in Frequency-Domain Fluorescence Lifetime Imaging of FRET Constructs

Linear Behavior of the Phase Lifetime in Frequency-Domain Fluorescence Lifetime Imaging of FRET Constructs

We utilize a cost-effective frequency-domain fluorescence lifetime imaging microscope to measure the phase lifetime of mTFP1 in mTFP1-mVenus fluorescence resonance energy transfer (FRET) constructs relevant to the VinTS molecular tension probe. Our data were collected at 15 modulation frequencies ω/...

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Bibliographic Details
Main Authors: Daniel Sumetsky, James Y. Jiang, Marina A. Ayad, Timothy Mahon, Audrey Menaesse, Marina M. Cararo-Lopes, Mihir V. Patel, Bonnie L. Firestein, Nada N. Boustany
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Physics
Subjects:
fluorescence lifetime
imaging microscopy
live cell assays
fluorescence resonance energy transfer
frequency domain
mTFP1
Online Access:https://www.frontiersin.org/articles/10.3389/fphy.2021.648016/full

Internet

https://www.frontiersin.org/articles/10.3389/fphy.2021.648016/full

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