Restoration of wild-type motility to flagellin-knockout Escherichia coli by varying promoter, copy number and induction strength in plasmid-based expression of flagellin

Flagellin is the major constituent of the flagellar filament and faithful restoration of wild-type motility to flagellin mutants may be beneficial for studies of flagellar biology and biotechnological exploitation of the flagellar system. However, gene complementation studies often fail to report wh...

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Bibliographic Details
Main Authors: Nicholas M. Thomson, Mark J. Pallen
Format: Article
Language:English
Published: Elsevier 2020-11-01
Series:Current Research in Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590262820300058