A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Abstract Background Recalcitrant nature is a major constraint for the in vitro regeneration and genetic transformation of leguminous species members. Therefore, an improved genetic transformation in horse gram has been developed via in planta method, in which Agrobacterium strain harboring binary ve...

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Bibliographic Details
Main Authors: Thomas Cheeran Amal, Palanisamy Karthika, Gurusamy Dhandapani, Subramaniam Selvakumar, Krishnan Vasanth
Format: Article
Language:English
Published: SpringerOpen 2020-03-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
Horse gram
In planta transformation
Optical cell density
Sonication
Vacuum infiltration
GUS expression
Online Access:http://link.springer.com/article/10.1186/s43141-020-00023-z

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http://link.springer.com/article/10.1186/s43141-020-00023-z

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