Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels

Summary: Transcription factor (TF) expression levels drive developmental programs, including cell fate and function, and their measurement by flow cytometry allows for robust downstream analysis. However, significant batch-to-batch variability between replicative experiments precludes direct compari...

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Bibliographic Details
Main Authors: Bryce A. Manso, Kay L. Medina
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001921